H2N2V1, Main, Exploration, bibRecord, 000238

Development of a Bead-Based Multiplex Assay for the Analysis of the Serological Response against the Six Pathogens HAV, HBV, HCV, CMV, T. gondii, and H. pylori

Identifieur interne : 000238 ( Main/Exploration ); précédent : 000237; suivant : 000239

Development of a Bead-Based Multiplex Assay for the Analysis of the Serological Response against the Six Pathogens HAV, HBV, HCV, CMV, T. gondii, and H. pylori

Auteurs : Angela Filomena [Allemagne] ; Frank Pessler [Allemagne] ; Manas Akmatov [Allemagne] ; Gérard Krause [Allemagne] ; Darragh Duffy ; Barbara G Rtner [Allemagne] ; Markus Gerhard [Allemagne] ; Matthew Albert ; Thomas Joos [Allemagne] ; Nicole Schneiderhan-Marra [Allemagne]

Source :

High-Throughput [ 2571-5135 ] ; 2017-12.

RBID : Hal:pasteur-01659434

English descriptors

mix :
- Helicobacter pylori, Toxoplasma gondii, cytomegalovirus (CMV), hepatitis, multi-pathogen assay, multiplex, seroprevalence, serotest.

Abstract

The spread of infectious diseases and vaccination history are common subjects of epidemiological and immunological research studies. Multiplexed serological assays are useful tools for assessing both current and previous infections as well as vaccination efficacy. We developed a serological multi-pathogen assay for hepatitis A, B and C virus, cytomegalovirus (CMV), Toxoplasma gondii, and Helicobacter pylori using a bead-based multiplex assay format. The multi-pathogen assay consisting of 15 antigens was utilized for the analysis of the serological response in elderly individuals of an influenza vaccination study (n = 34). The technical assay validation revealed a mean intra-assay precision of coefficient of variation (CV) = 3.2 ± 1.5% and a mean inter-assay precision of CV = 8.2 ± 5.3% across all 15 antigens and all tested samples, indicating a robust test system. Furthermore, the assay shows high sensitivities (ranging between 94% and 100%) and specificities (ranging between 93% and 100%) for the different pathogens. The highest seroprevalence rates in our cohort were observed for hepatitis A virus (HAV; 73.5%), followed by CMV (70.6%), T. gondii (67.6%) and H. pylori (32.4%). Seroprevalences for hepatitis B virus (HBV, 8.8%) and hepatitis C virus (HCV, 0%) were low. The seroprevalences observed in our study were similar to those from other population-based studies in Germany. In summary, we conclude that our multiplex serological assay represents a suitable tool for epidemiological studies.

Url:

https://hal-pasteur.archives-ouvertes.fr/pasteur-01659434

DOI: 10.3390/ht6040014

Affiliations:

Allemagne

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000057
to stream Hal, to step Curation: 000057
to stream Hal, to step Checkpoint: 000046
to stream Main, to step Merge: 000238
to stream Main, to step Curation: 000238

Le document en format XML

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<front><div type="abstract" xml:lang="en">              <p>The spread of infectious diseases and vaccination history are common subjects of epidemiological and immunological research studies. Multiplexed serological assays are useful tools for assessing both current and previous infections as well as vaccination efficacy. We developed a serological multi-pathogen assay for hepatitis A, B and C virus, cytomegalovirus (CMV), Toxoplasma gondii, and Helicobacter pylori using a bead-based multiplex assay format. The multi-pathogen assay consisting of 15 antigens was utilized for the analysis of the serological response in elderly individuals of an influenza vaccination study (n = 34). The technical assay validation revealed a mean intra-assay precision of coefficient of variation (CV) = 3.2 ± 1.5% and a mean inter-assay precision of CV = 8.2 ± 5.3% across all 15 antigens and all tested samples, indicating a robust test system. Furthermore, the assay shows high sensitivities (ranging between 94% and 100%) and specificities (ranging between 93% and 100%) for the different pathogens. The highest seroprevalence rates in our cohort were observed for hepatitis A virus (HAV; 73.5%), followed by CMV (70.6%), T. gondii (67.6%) and H. pylori (32.4%). Seroprevalences for hepatitis B virus (HBV, 8.8%) and hepatitis C virus (HCV, 0%) were low. The seroprevalences observed in our study were similar to those from other population-based studies in Germany. In summary, we conclude that our multiplex serological assay represents a suitable tool for epidemiological studies.</p>
            </div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
</country>
</list>
<tree><noCountry><name sortKey="Albert, Matthew" sort="Albert, Matthew" uniqKey="Albert M" first="Matthew" last="Albert">Matthew Albert</name>
<name sortKey="Duffy, Darragh" sort="Duffy, Darragh" uniqKey="Duffy D" first="Darragh" last="Duffy">Darragh Duffy</name>
</noCountry>
<country name="Allemagne"><noRegion><name sortKey="Filomena, Angela" sort="Filomena, Angela" uniqKey="Filomena A" first="Angela" last="Filomena">Angela Filomena</name>
</noRegion>
<name sortKey="Akmatov, Manas" sort="Akmatov, Manas" uniqKey="Akmatov M" first="Manas" last="Akmatov">Manas Akmatov</name>
<name sortKey="G Rtner, Barbara" sort="G Rtner, Barbara" uniqKey="G Rtner B" first="Barbara" last="G Rtner">Barbara G Rtner</name>
<name sortKey="Gerhard, Markus" sort="Gerhard, Markus" uniqKey="Gerhard M" first="Markus" last="Gerhard">Markus Gerhard</name>
<name sortKey="Joos, Thomas" sort="Joos, Thomas" uniqKey="Joos T" first="Thomas" last="Joos">Thomas Joos</name>
<name sortKey="Krause, Gerard" sort="Krause, Gerard" uniqKey="Krause G" first="Gérard" last="Krause">Gérard Krause</name>
<name sortKey="Pessler, Frank" sort="Pessler, Frank" uniqKey="Pessler F" first="Frank" last="Pessler">Frank Pessler</name>
<name sortKey="Schneiderhan Marra, Nicole" sort="Schneiderhan Marra, Nicole" uniqKey="Schneiderhan Marra N" first="Nicole" last="Schneiderhan-Marra">Nicole Schneiderhan-Marra</name>
</country>
</tree>
</affiliations>
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Development of a Bead-Based Multiplex Assay for the Analysis of the Serological Response against the Six Pathogens HAV, HBV, HCV, CMV, T. gondii, and H. pylori

Development of a Bead-Based Multiplex Assay for the Analysis of the Serological Response against the Six Pathogens HAV, HBV, HCV, CMV, T. gondii, and H. pylori

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